Plant disease control composition and plant disease control method

ABSTRACT

A composition for controlling plant diseases comprising Paecilomyces fumosoroseus, and one or more nematicidal active ingredients selected from specific groups has an excellent controlling effect on plant diseases.

CROSS-REFERENCE TO RELATED APPLICATION

This application is a Divisional application of co-pending applicationSer. No. 15/100,182, filed on May 27, 2016, which is the National Phaseunder 35 U.S.C. § 371 of International Application No.PCT/JP2014/081916, filed on Nov. 26, 2014, which claims the benefitunder 35 U.S.C. § 119(a) to Patent Application No. 2013-248951, filed inJapan on Dec. 2, 2013 and Patent Application No. 2014-182036, filed inJapan on Sep. 8, 2014, all of which are hereby expressly incorporated byreference into the present application.

TECHNICAL FIELD

The present invention relates to a composition for controlling plantdiseases and a method for controlling plant diseases.

BACKGROUND ART

Conventionally, many compounds (for example, refer to Non-PatentDocument 1) and fungicides derived from microorganisms (for example,refer to Non-Patent Documents 2 and 3) have been used as an activeingredient of a plant disease controlling agent.

PRIOR ART DOCUMENTS Non-Patent Documents

Non-Patent Document 1: The Pesticide Manual-16th edition (published byBCPC); ISBN 978-1-901396-86-7

Non-Patent Document 2: SHIBUYA INDEX (Index of Pesticides)-2012-16thedition (Zenkoku Nokyo Kyouiku Kyoukai); ISBN 978-4-88137-162-6

Non-Patent Document 3: Journal of Nematology 31 (1): 54-61. 1999.

DISCLOSURE OF THE INVENTION

An object of the present invention is to provide a composition forcontrolling plant diseases having an excellent plant disease controllingeffect, and a method for controlling plant diseases.

As a result of studying to find a composition for controlling plantdiseases having an excellent plant disease controlling effect, thepresent inventors have found that a composition containing Paecilomycesfumosoroseus, and one or more nematicidal active ingredients selectedfrom the group consisting of Group (A1) and Group (A2) has an excellentcontrolling effect on plant diseases.

More specifically, the present invention is as described below.

[1] A composition for controlling plant diseases comprising Paecilomycesfumosoroseus, and one or more nematicidal active ingredients selectedfrom the group consisting of Group (A1) and Group (A2).Group (A1): group consisting of aldicarb, thiodicarb, carbofuran,carbosulfan, dimethoate, fenamiphos, imicyafos, fensulfothion, terbufos,fosthiazate, dichlofenthion, isamidofos, fosthietan, isazofos,ethoprophos, cadusafos, phorate, thionazin, triazophos, diamidafos,phosphamidon, fluensulfone, MEP (fenitrothion), and fluopyram.Group (A2): group consisting of Harpin protein, Pasteuria nishizawae,Pasteuria penetrans, Pasteuria usgae, Myrothecium verrucaria,Burkholderia cepacia, Bacillus amyloliquefaciens, Bacillus firmus,Bacillus subtillis, Bacillus pumulis, Trichoderma harzianum,Verticillium chlamydosporum, and Bacillus megaterium.[2] The composition for controlling plant diseases according to [1],wherein the nematicidal active ingredients are one or more nematicidalactive ingredients selected from Group (A1), and the ratio of the number(CFU) of Paecilomyces fumosoroseus to the amount (g) of the nematicidalactive ingredients selected from Group (A1) is number of bacteria(CFU):amount (g)=10⁷:1 to 10¹⁷:1.[3] The composition for controlling plant diseases according to [1],wherein the nematicidal active ingredients are one or more nematicidalactive ingredients selected from Group (A2), and the ratio of the numberof Paecilomyces fumosoroseus to the amount of the nematicidal activeingredients selected from Group (A2) is number of bacteria (CFU):numberof bacteria (CFU)=1:10⁵ to 10⁵:1.[4] A method for controlling plant diseases comprising applying aneffective amount of the composition for controlling plant diseases asdefined in any one of [1] to [3] to a seed, a plant, or soil forcultivating a plant.[5] A plant seed treated with an effective amount of the composition forcontrolling plant diseases as defined in any one of [1] to [3].[6] The composition for controlling plant diseases according to any oneof [1] to [3], wherein the plant disease is a soil-borne disease.[7] The controlling method according to [4], wherein the plant diseaseis a soil-borne disease.[8] The plant seed according to [5] , wherein the plant disease is asoil-borne disease.

MODE FOR CARRYING OUT THE INVENTION

The composition for controlling plant diseases of the present inventioncontains Paecilomyces fumosoroseus, and one or more nematicidal activeingredients selected from the group consisting of Group (A1) and Group(A2).

Paecilomyces fumosoroseus used in the composition for controlling plantdiseases of the present invention is a filamentous fungus described inPest Management Science (2012) , 68(4), 573-579 and Crop Protection 20(2001) 767-778, and includes strain FE9901 (NOFLY (registeredtrademark), accession number: NITE BP-01876, original deposition date:Jun. 18, 2014, deposited to National Institute of Technology andEvaluation, NITE Patent Microorganisms Depositary, room 122, 2-5-8,Kazusakamatari, Kisarazu-shi, Chiba, Japan 292-0818), strain Apopka 97(Preferred (PreFeRal (registered trademark), PFR-97 (registeredtrademark)), and the like, and can be obtained from commerciallyavailable formulations such as NOFLY (registered trademark) and PreFeRal(registered trademark) or isolated and cultured by a known method.

The nematicidal active ingredient used in the composition forcontrolling plant diseases of the present invention represents acomponent that controls nematodes. Specifically, the nematicidal activeingredient includes one or more components selected from the groupconsisting of Group (A1) and Group (A2).

Among Group (A1), aldicarb, thiodicarb, carbofuran and carbosulfan areknown as carbamate-based components, and dimethoate, fenamiphos,imicyafos, fensulfothion, terbufos, fosthiazate, dichlofenthion,isamidofos, fosthietan, isazofos, ethoprophos, cadusafos, phorate,thionazin, triazophos, diamidafos, phosphamidon, fluensulfone and MEPare known as organophosphorus components.

Also, the components of Group (A2) are known as bacteria derived frommicroorganisms.

Aldicarb, thiodicarb, carbofuran, carbosulfan, dimethoate, fenamiphos,imicyafos, fensulfothion, terbufos, fosthiazate, dichlofenthion,isamidofos, fosthietan, isazofos, ethoprophos, cadusafos, phorate,thionazin, triazophos, diamidafos, phosphamidon, fluensulfone, MEP,fluopyram, Harpin protein, Pasteuria nishizawae, Pasteuria penetrans,Pasteuria usgae, Myrothecium verrucaria, Burkholderia cepacia, Bacillusamyloliquefaciens, Bacillus firmus, Bacillus subtillis, Bacilluspumulis, Trichoderma harzianum, Verticillium chlamydosporum, Bacillusmegaterium (NoNem: tradename) are all known components, and described,for example, in pages 24, 1115, 158, 160, 369, 454, 639, 1211, 1081,573, 323, 1216, 1213, 1216, 437, 148, 883, 1230, 1143, 1203, 887, 513,465 and 528 of “The Pesticide Manual-16th edition (published by BCPC) ;ISBN 978-1-901396-86-7”, and pages 92, 72, 72, 72, 74, 72, 162, 71, 162,162, 166, and 75 of “SHIBUYA INDEX (Index of Pesticides) -2012-16thedition (Zenkoku Nokyo Kyouiku Kyoukai); ISBN 978-4-88137-162-6”, andJournal of Nematology 31(1): 54-61. 1999., and can be obtained fromcommercially available formulations, produced or isolated and culturedby a known method.

The content ratio of Paecilomyces fumosoroseus to the nematicidal activeingredients in the composition for controlling plant diseases of thepresent invention is not particularly limited, but typically, the ratioof the number of Paecilomyces fumosoroseus to the amount of thenematicidal active ingredients selected from Group (A1) is number ofbacteria (CFU):amount (g)=10⁷:1 to 10¹⁷:1, and preferably number ofbacteria (CFU):amount (g)=1:10⁸ to 10¹³:1, and the ratio of the numberof Paecilomyces fumosoroseus to the amount of the nematicidal activeingredients selected from Group (A2) is number of bacteria (CFU):numberof bacteria (CFU)=1:10⁵ to 10⁵:1, and preferably number of bacteria(CFU):number of bacteria (CFU)=1:10² to 10²:1

The composition for controlling plant diseases of the present inventionmay be a simple mixture of Paecilomyces fumosoroseus and one or morenematicidal active ingredients selected from the group consisting ofGroup (A1) and Group (A2) , but typically, obtained by mixingPaecilomyces fumosoroseus, one or more nematicidal active ingredientsselected from the group consisting of Group (A1) and Group (A2) , and aninert carrier such as a solid carrier or a liquid carrier, and adding asurfactant or other auxiliaries for formulation as necessary, to beformulated into emulsifiable oil formulations, emulsions, flowableagents, wettable powder, wettable granules, dust formulations, granules,and the like. Also, the composition for controlling plant diseases ofthe present invention can be used as it is or as a seed treatment agentof the present invention by adding other inert component.

In the composition for controlling plant diseases of the presentinvention, the total content of Paecilomyces fumosoroseus and one ormore nematicidal active ingredients selected from the group consistingof Group (A1) and Group (A2) is typically 0.1 to 99% by weight, andpreferably 0.2 to 90% by weight.

Examples of the solid carrier used upon formulation include fine powdersor granules of minerals such as kaolin clay, attapulgite clay,bentonite, montmorillonite, acid white clay, pyrophyllite, talc,diatomaceous earth and calcite, natural organic materials such as corncob powder and walnut husk powder, synthetic organic materials such asurea, salts such as calcium carbonate and ammonium sulfate, syntheticinorganic materials such as synthetic hydrated silicon oxide, and thelike. Examples of the liquid carrier include aromatic hydrocarbons suchas xylene, alkylbenzene and methylnaphthalene, alcohols such as2-propanol, ethylene glycol, propylene glycol and ethylene glycolmonoethyl ether, ketones such as acetone, cyclohexanone and isophorone,vegetable oils such as soybean oil and cotton seed oil, and petroleumaliphatic hydrocarbons, esters, dimethylsulfoxide, acetonitrile, andwater.

Examples of the surfactant include anionic surfactants such as alkylsulfonate ester salts, alkylaryl sulfonate salts, dialkyl sulfosuccinatesalts, polyoxyethylene alkyl aryl ether phosphate ester salts,lignosulfonate salts and naphthalene sulfonate formaldehydepolycondensates, nonionic surfactants such as polyoxyethylene alkyl arylethers, polyoxyethylene alkylpolyoxypropylene block copolymers andsorbitan fatty acid esters, and cationic surfactants such asalkyltrimethylammonium salts.

Examples of the other auxiliaries for formulation include water-solublepolymers such as polyvinyl alcohol and polyvinyl pyrrolidone,polysaccharides such as arabic gum, alginic acid and salts thereof, CMC(carboxymethyl cellulose) and xanthan gum, inorganic materials such asaluminum magnesium silicate and alumina sol, preservatives, colorantsand stabilizers such as PAP (isopropyl acid phosphate) and BHT.

The composition for controlling plant diseases of the present inventioncan be also prepared by formulating Paecilomyces fumosoroseus and one ormore nematicidal active ingredients selected from the group consistingof Group (A1) and Group (A2) respectively by the methods describedabove, then mixing each of the formulations diluted with water asnecessary, or their diluents.

The composition for controlling plant diseases of the present inventionmay further contain one or more fungicides.

Examples of the plant disease that can be controlled by the presentinvention include the following.

Rice diseases: Magnaporthe grisea, Cochliobolus miyabeanus, Rhizoctoniasolani, and Gibberella fujikuroi.

Wheat diseases: Erysiphegraminis, Fusariumgraminearum, F. avenacerum, F.culmorum, Microdochium nivale, Puccinia striiformis, P. graminis, P.recondita, Micronectriella nivale, Typhula spp., Ustilago tritici,Tilletia caries, Pseudocercosporella herpotrichoides, Mycosphaerellagraminicola, Stagonospora nodorum, and Pyrenophora tritici-repentis.

Barley diseases: Erysiphe graminis, Fusariumgraminearum, F. avenacerum,F. culmorum, Microdochium nivale, Puccinia striiformis, P. graminis, P.hordei, Ustilago nuda, Rhynchosporium secalis, Pyrenophora teres,Cochliobolus sativus, Pyrenophora graminea, and Rhizoctonia solani bygenus Rhizoctonia.

Corn diseases: Ustilago maydis, Cochliobolus heterostrophus,Gloeocercospora sorghi, Puccinia polysora, Cercospora zeae-maydis, andRhizoctonia solani by genus Rhizoctonia.

Citrus diseases: Diaporthe citri, Elsinoe fawcetti, Penicilliumdigitatum, P. italicum, Phytophthora parasitica, and Phytophthoracitrophthora.

Apple diseases: Monilinia mali, Valsa ceratosperma, Podosphaeraleucotricha, Alternaria alternata apple pathotype, Venturia inaequalis,Colletotrichum acutatum, Phytophtora cactorum, Diplocarpon mali,Botryosphaeria berengeriana, and Helicobasidium mompa.

Pear diseases: Venturia nashicola, V. pirina, Alternaria alternataJapanese pear pathotype, Gymnosporangium haraeanum, and Phytophtoracactorum.

Peach diseases: Monilinia fructicola, Cladosporium carpophilum, andPhomopsis spp.

Grape diseases: Elsinoe ampelina, Glomerella cingulata, Uncinulanecator, Phakopsora ampelopsidis, Guignardia bidwellii, and Plasmoparaviticola.

Persimmon diseases: Gloeosporium kaki, Cercospora kaki, andMycosphaerella nawae.

Gourd diseases: Colletotrichum lagenarium, Sphaerotheca fuliginea,Mycosphaerella melonis, Fusarium oxysporum, Pseudoperonospora cubensis,Phytophthora spp., and Pythium spp.

Tomato diseases: Alternaria solani, Cladosporium fulvum, Phytophthorainfestans, Ralstonia solanacearum, and Fusarium oxysporum f. sp.lycopersici.

Eggplant diseases: Phomopsis vexans, Erysiphe cichoracearum, Ralstoniasolanacearum, and Verticillium dahliae.

Cruciferous vegetable diseases: Alternaria japonica, Cercosporellabrassicae, Plasmodiophora brassicae, and Peronospora parasitica.

Welsh onion diseases: Puccinia allii and Peronospora destructor.

Soybean diseases: Cercospora kikuchii, Elsinoe glycines, Diaporthephaseolorum var. sojae, Septoria glycines, Cercospora sojina, Phakopsorapachyrhizi, Phytophthora sojae, and Rhizoctonia solani by genusRhizoctonia.

Kidney bean diseases: Colletotrichum lindemthianum.

Peanut diseases: Cercospora personata, Cercospora arachidicola, andSclerotium rolfsii.

Pea diseases: Erysiphe pisi and Fusarium solani F. sp. pisi

Potato diseases: Alternaria solani, Phytophthora infestans, Phytophthoraerythroseptica, Spongospora subterranean F. sp. subterranea, Rhizoctoniasolani, and Ralstonia solanacearum.

Strawberry diseases: Sphaerotheca humuli and Glomerella cingulata.

Tea diseases: Exobasidium reticulatum, Elsinoe leucospila,Pestalotiopsis spp., and Colletotrichum theae-sinensis.

Tobacco diseases: Alternaria longipes, Erysiphe cichoracearum,Colletotrichum tabacum, Peronospora tabacina, and Phytophthoranicotianae.

Rapeseed diseases: Sclerotinia sclerotiorum, and Rhizoctonia solani bygenus Rhizoctonia.

Cotton diseases: Rhizoctonia solani by genus Rhizoctonia.

Sugar beet diseases: Cercospora beticola, Rhizoctonia solani,Rhizoctonia solani, and Aphanomyces cochlioides.

Rose diseases: Diplocarpon rosae, Sphaerotheca pannosa, and Peronosporasparsa.

Crysanthemums and Compositae vegetable diseases: Bremia lactucae,Septoria chrysanthemi-indici, and Puccinia horiana.

Various plant diseases: diseases caused by genus Pythium (Pythiumdebarianum, Pythium graminicola, Pythium irregulare, Pythiumultimum),Botrytis cinerea, Sclerotinia sclerotiorum, and Sclerotium rolfsii.

Japanese radish diseases: Alternaria brassicicola.

Lawn diseases: Sclerotinia homeocarpa, and Rhizoctonia solani.

Banana diseases: Mycosphaerella fijiensis and Mycosphaerella musicola.

Sunflower diseases: Plasmopara halstedii.

Seed-borne diseases of various plants or diseases at the initial stageof growth, caused by genus Aspergillus, genus Penicillium, genusFusarium, genus Gibberella, genus Tricoderma, genus Thielaviopsis, genusRhizopus, genus Mucor, genus Corticium, genus Phoma, genus Rhizoctonia,genus Diplodia, and the like.

Viral diseases of various plants mediated by genus Polymixa, genusOlpidium or the like.

In the present invention, the soil-borne disease represents a diseasetransmitted via soil where plant pathogens are present, and examplesinclude Ralstonia solanacearum, Plasmodiophora brassicae, Phytophthorasojae, Fusarium oxysporum f.sp. lycopersici, and chlorosis. Thecomposition of the present invention is particularly effective onsoil-borne disease.

Plant diseases can be controlled by applying effective amounts ofPaecilomyces fumosoroseus and one or more nematicidal active ingredientsselected from the group consisting of Group (A1) and Group (A2) to aplant or to such a place (plant, soil, etc.) where the plant pathogensinhabit or may inhabit.

Plant diseases can be controlled by applying effective amounts ofPaecilomyces fumosoroseus and one or more nematicidal active ingredientsselected from the group consisting of Group (A1) and Group (A2) to aseed, a plant, or a soil for cultivating a plant. Examples of the plantwhich is the object of the application include foliages of plant and thelike, and the seeds of plant also include bulbs of plant. As usedherein, the bulb means a bulb, corm, rhizome, stem tuber, root tuber andrhizophore.

When applied to a plant, a soil for cultivating a plant, or the like,Paecilomyces fumosoroseus and one or more nematicidal active ingredientsselected from the group consisting of Group (A1) and Group (A2) may beseparately applied for the same period, but they are typically appliedas the composition for controlling plant diseases of the presentinvention for simplicity of the application.

Examples of the controlling method of the present invention specificallyinclude treatment of foliage of plants, such as foliage spraying;treatment of cultivation lands of plants, such as soil treatment;treatment of seeds, such as seed sterilization and seed coating;treatment of bulbs such as seed tuber; and the like.

Examples of the treatment of plants in the controlling method of thepresent invention include a treatment method of allowing the compositionof the present invention directly absorb to plants beforetransplantation and treatment methods of applying the composition of thepresent invention to surfaces of plants, such as foliage spraying andtrunk spraying. Examples of the treatment method of allowing thecomposition of the present invention directly absorb to plants beforetransplantation include a method of soaking entire plants or roots in aliquid containing the composition of the present invention, and a methodof adhering a formulation of the composition of the present inventionobtained by using a solid carrier such as a mineral powder, to theroots.

Examples of the method of treating cultivation lands of plants with thecomposition of the present invention in the controlling method of thepresent invention include spraying onto the soil, soil incorporation,and perfusion of a chemical liquid into the soil (irrigation of chemicalliquid, soil injection, and dripping of chemical liquid). Examples ofthe place to be treated include planting hole, furrow, around a plantinghole, around a furrow, entire surface of cultivation lands, the partsbetween the soil and the plant, area between roots, area beneath thetrunk, main furrow, growing soil, seedling raising box, seedling raisingtray, seedbed, and the like. Examples of the treating period includebefore seeding, at the time of seeding, immediately after seeding,raising period, before settled planting, at the time of settledplanting, growing period after settled planting, and the like. Also, inthe treatment of cultivation lands of plants, a solid fertilizer such asa paste fertilizer containing an active ingredient may be applied to thesoil. When treating an active ingredient to the cultivation land of theplant, the active ingredient may be also treated to the plant. Also, thecomposition of the present invention may be mixed in an irrigationliquid, and examples include injecting to irrigation facilities (such asirrigation tube, irrigation pipe and sprinkler), mixing into theflooding liquid between furrows, mixing into a water culture medium, andthe like. Alternatively, an irrigation liquid is mixed with activeingredients in advance and, for example, can be treated using anappropriate irrigating method including the irrigating method mentionedabove and the other methods such as sprinkling and flooding.

Examples of the method of treating seeds with the composition of thepresent invention in the controlling method of the present inventioninclude a method for treating seeds to be protected from plant diseaseswith the composition for controlling plant diseases of the presentinvention, and specific examples include a spraying treatment in which asuspension of the composition for controlling plant diseases of thepresent invention is atomized and sprayed on the seed surface; asmearing treatment in which a wettable powder, an emulsion or a flowableagent of the composition for controlling plant diseases of the presentinvention is applied to seeds with a small amount of water added orwithout dilution; an immersing treatment in which seeds are immersed ina solution of the composition for controlling plant diseases of thepresent invention for a certain period of time; film coating treatment;and pellet coating treatment.

When treating a plant, or a soil for cultivating a plant withPaecilomyces fumosoroseus and one or more nematicidal active ingredientsselected from the group consisting of Group (A1) and Group (A2), thetreatment amount may be changed depending on the kind of the plant to betreated, the kind and the occurring frequency of the plant diseases tobe controlled, formulation form, treatment period, climatic conditionand the like, but the total content of Paecilomyces fumosoroseus and oneor more nematicidal active ingredients selected from the groupconsisting of Group (A1) and Group (A2) (hereinafter referred to as “theamount of the active ingredients”.) per 10,000 m² is typically 1 to5,000 g and preferably 2 to 500 g.

The emulsion, wettable powder, flowable agent and the like are typicallydiluted with water, and then sprinkled for the treatment. In these case,the concentration of the amount of the active ingredients is typically0.0001 to 3% by weight and preferably 0.0005 to 1% by weight. The powderagent, granules and the like are typically used for the treatmentwithout being diluted.

In the treatment of seeds, the amount of the active ingredients istypically 0.001 to 10 g, and preferably 0.01 to 3 g per 1 kg of seeds.

The control method of the present invention can be used in agriculturallands such as fields, paddy fields, lawns and orchards or innon-agricultural lands.

Also, the present invention can be used for controlling plant diseasesof agricultural lands without adversely affecting the plant and thelike, in the agricultural lands for cultivating the following “plant”and the like.

Crops: corn, rice, wheat, barley, rye, oat, sorghum, cotton, soybean,peanut, sarrazin, sugar beet, rapeseed, sunflower, sugar cane, tobacco,etc.; Vegetables: Solanaceae vegetables (eggplant, tomato, green pepper,hot pepper, potato, etc.), Cucurbitaceae vegetables (cucumber, pumpkin,zucchini, watermelon, melon, squash, etc.), Cruciferae vegetables(Japanese radish, turnip, turnip, horseradish, kohlrabi, Chinesecabbage, cabbage, brown mustard, broccoli, cauliflower, etc.),Compositae vegetables (burdock, garland chrysanthemum, artichoke,lettuce, etc.), Liliaceae vegetables (Welsh onion, onion, garlic,asparagus, etc.), Umbelliferae vegetables (carrot, parsley, celery,parsnip, etc.), Chenopodiaceae vegetables (spinach, Swiss chard, etc.),Labiatae vegetables (Japanese mint, mint, basil, etc.), strawberry,sweet potato, yam, aroid, etc.;

Flowers;

Ornamental foliage plants;

Lawn;

Fruit trees: pomaceous fruits (apple, common pear, Japanese pear,Chinese quince, quince, etc.), stone fleshy fruits (peach, plum,nectarine, Japanese plum, cherry, apricot, prune, etc.), citrus plants(Satsuma mandarin, orange, lemon, lime, grapefruits, etc.), nuts(chestnut, walnut, hazel nut, almond, pistachio, cashew nut, macadamianut, etc.), berry fruits (blueberry, cranberry, blackberry, raspberry,etc.), grape, persimmon, olive, loquat, banana, coffee, date, coconut,etc.;

Trees other than fruit trees: tea, mulberry, flowering plants, Jatrophacurucas, street trees (ash tree, birch, dogwood, eucalyptus, ginkgo,lilac, maple tree, oak, poplar, cercis, Chinese sweet gum, plane tree,zelkova, Japanese arborvitae, fir tree, Japanese hemlock, needlejuniper, pine, spruce, yew), etc.

Particularly, the control method of the present invention can be used tocontrol diseases in agricultural lands for cultivating corn, rice,wheat, barley, sorghum, cotton, soybean, beet, rapeseed, lawn or potato.

The above plants also contain genetically modified plants.

EXAMPLES

Hereinbelow, the present invention will be further described in detailby formulation examples and test examples. However, the presentinvention is not limited to the following examples. In the followingexamples, the part means part by weight unless otherwise specificallynoted.

Formulation Example 1

Two parts of Paecilomyces fumosoroseus (strain FE9901) spore, 2 parts offluopyram, 72 parts of SALACOS (registered trademark) 5408 (manufacturedby The Nisshin OilliO Group, Ltd.), 17 parts of COSMOL (registeredtrademark) 44V (manufactured by The Nisshin OilliO Group, Ltd.) and 10parts of Pegnol (registered trademark) 24-O (manufactured by TOHOChemical Industry Co., Ltd.) are well mixed to obtain a formulation.

Formulation Example 2

Two parts of Paecilomyces fumosoroseus (strain FE9901) spore, 2 parts ofMEP (fenitrothion), 72 parts of SALACOS (registered trademark) 5408(manufactured by The Nisshin OilliO Group, Ltd.), 17 parts of COSMOL(registered trademark) 44V (manufactured by The Nisshin OilliO Group,Ltd.) and 10 parts of Pegnol (registered trademark) 24-O (manufacturedby TOHO Chemical Industry Co., Ltd.) are well mixed to obtain aformulation.

Formulation Example 3

Two parts of Paecilomyces fumosoroseus (strain FE9901) spore, 2 parts offluensulfone, 72 parts of SALACOS (registered trademark) 5408(manufactured by The Nisshin OilliO Group, Ltd.), 17 parts of COSMOL(registered trademark) 44V (manufactured by The Nisshin OilliO Group,Ltd.) and 10 parts of Pegnol (registered trademark) 24-0 (manufacturedby TOHO Chemical Industry Co., Ltd.) are well mixed to obtain aformulation.

Formulation Example 4

Two parts of Paecilomyces fumosoroseus (strain FE9901) spore, 2 parts ofaldicarb, 72 parts of SALACOS (registered trademark) 5408 (manufacturedby The Nisshin OilliO Group, Ltd.), 17 parts of COSMOL (registeredtrademark) 44V (manufactured by The Nisshin OilliO Group, Ltd.) and 10parts of Pegnol (registered trademark) 24-O (manufactured by TOHOChemical Industry Co., Ltd.) are well mixed to obtain a formulation.

Formulation Example 5

Two parts of Paecilomyces fumosoroseus (strain FE9901) spore, 2 parts ofethoprophos, 72 parts of SALACOS (registered trademark) 5408(manufactured by The Nisshin OilliO Group, Ltd.), 17 parts of COSMOL(registered trademark) 44V (manufactured by The Nisshin OilliO Group,Ltd.) and 10 parts of Pegnol 2 (registered trademark) 4-O (manufacturedby TOHO Chemical Industry Co., Ltd.) are well mixed to obtain aformulation.

Formulation Example 6

Two parts of Paecilomyces fumosoroseus (strain FE9901) spore, 2 parts offenamiphos, 72 parts of SALACOS (registered trademark) 5408(manufactured by The Nisshin OilliO Group, Ltd.), 17 parts of COSMOL(registered trademark) 44V (manufactured by The Nisshin OilliO Group,Ltd.) and 10 parts of Pegnol (registered trademark) 24-O (manufacturedby TOHO Chemical Industry Co., Ltd.) are well mixed to obtain aformulation.

Formulation Example 7

Two parts of Paecilomyces fumosoroseus (strain FE9901) spore, 2 parts offosthiazate, 72 parts of SALACOS (registered trademark) 5408(manufactured by The Nisshin OilliO Group, Ltd.), 17 parts of COSMOL(registered trademark) 44V (manufactured by The Nisshin OilliO Group,Ltd.) and 10 parts of Pegnol (registered trademark) 24-O (manufacturedby TOHO Chemical Industry Co., Ltd.) are well mixed to obtain aformulation.

Formulation Example 8

Two parts of Paecilomyces fumosoroseus (strain FE9901) spore, 2 parts ofcadusafos, 72 parts of SALACOS (registered trademark) 5408 (manufacturedby The Nisshin OilliO Group, Ltd.), 17 parts of COSMOL (registeredtrademark) 44V (manufactured by The Nisshin OilliO Group, Ltd.) and 10parts of Pegnol (registered trademark) 24-0 (manufactured by TOHOChemical Industry Co., Ltd.) are well mixed to obtain a formulation.

Formulation Example 9

Two parts of Paecilomyces fumosoroseus (strain FE9901) spore, 2 parts ofimicyafos, 72 parts of SALACOS (registered trademark) 5408 (manufacturedby The Nisshin OilliO Group, Ltd.), 17 parts of COSMOL (registeredtrademark) 44V (manufactured by The Nisshin OilliO Group, Ltd.) and 10parts of Pegnol (registered trademark) 24-O (manufactured by TOHOChemical Industry Co., Ltd.) are well mixed to obtain a formulation.

Formulation Example 10

Two parts of Paecilomyces fumosoroseus (strain FE9901) spore, 2 parts ofterbufos, 72 parts of SALACOS (registered trademark) 5408 (manufacturedby The Nisshin OilliO Group, Ltd.), 17 parts of COSMOL (registeredtrademark) 44V (manufactured by The Nisshin OilliO Group, Ltd.) and 10parts of Pegnol (registered trademark) 24-O (manufactured by TOHOChemical Industry Co., Ltd.) are well mixed to obtain a formulation.

Formulation Example 11

Two parts of Paecilomyces fumosoroseus (strain FE9901) spore, 2 parts ofBacillus firmus, 72 parts of SALACOS (registered trademark) 5408(manufactured by The Nisshin OilliO Group, Ltd.), 17 parts of COSMOL(registered trademark) 44V (manufactured by The Nisshin OilliO Group,Ltd.) and 10 parts of Pegnol (registered trademark) 24-O (manufacturedby TOHO Chemical Industry Co., Ltd.) are well mixed to obtain aformulation.

Formulation Example 12

Two parts of Paecilomyces fumosoroseus (strain FE9901) spore, 2 parts ofPasteuria usgae, 72 parts of SALACOS (registered trademark) 5408(manufactured by The Nisshin OilliO Group, Ltd.), 17 parts of COSMOL(registered trademark) 44V (manufactured by The Nisshin OilliO Group,Ltd.) and 10 parts of Pegnol (registered trademark) 24-O (manufacturedby TOHO Chemical Industry Co., Ltd.) are well mixed to obtain aformulation.

Formulation Example 13

Two parts of Paecilomyces fumosoroseus (strain FE9901) spore, 2 parts ofPasteuria nishizawae, 72 parts of SALACOS (registered trademark) 5408(manufactured by The Nisshin OilliO Group, Ltd.), 17 parts of COSMOL(registered trademark) 44V (manufactured by The Nisshin OilliO Group,Ltd.) and 10 parts of Pegnol (registered trademark) 24-O (manufacturedby TOHO Chemical Industry Co., Ltd.) are well mixed to obtain aformulation.

Formulation Example 14

Two parts of Paecilomyces fumosoroseus (strain FE9901) spore, 2 parts ofBacillus megaterium, 72 parts of SALACOS (registered trademark) 5408(manufactured by The Nisshin OilliO Group, Ltd.), 17 parts of COSMOL(registered trademark) 44V (manufactured by The Nisshin OilliO Group,Ltd.) and 10 parts of Pegnol (registered trademark) 24-O (manufacturedby TOHO Chemical Industry Co., Ltd.) are well mixed to obtain aformulation.

Test Example 1

Seventy-five milliliters of a suspension (1×10⁶ CFU/ml) of Paecilomycesfumosoroseus (strain FE9901, accession number: NITE BP-01876) spore anda prescribed amount of nematicidal active ingredients were treated to apot with a radius of 9 cm filled with Ralstoniasolanacearum-contaminated soil, and well mixed. Four days after chemicaltreatment, tomato seedlings were transplanted to this pot. One weekafter transplantation, a Ralstonia solanacearum suspension wasinoculated to the pot by drench, and tomato was cultivated in aglasshouse, at a daytime room temperature of 30° C. and a night roomtemperature of 20° C. Thirty-three days after transplantation, thedisease index was assessed from the degree of wilting of tomato, and thedisease severity was calculated by the following equation, based on thedisease index. The disease severity is defined as a disease severity oftreated plants.

Tomato was cultivated in the same manner as described above, except fornot treating Paecilomyces fumosoroseus and nematicidal activeingredients, and the disease severity was calculated. The diseaseseverity is defined as a disease severity of untreated plants.

The controlling value was calculated from the disease severity oftreated plants and the disease severity of untreated plants.

Disease index

-   -   0: no symptom of disease is found    -   1: wilting of the shoot apices is observed    -   2: wilting of the leaves is observed    -   3: the whole plant is wilted    -   4: the plant withers and dies

Disease severity=100×[{1×(number of plant with disease index of1)+2×(number of plant with disease index of 2)+3×(number of plant withdisease index of 3)+4×(number of plant with disease index of4)}/{4x×(total number of plant)}]

Controlling value (%)=100×(A−B)/A

A: disease severity of untreated plants

B: disease severity of treated plants

The result is shown in Table 1. In the table, “−” represents the case ofnot containing Paecilomyces fumosoroseus, and “+” represents the case ofcontaining Paecilomyces fumosoroseus (3×10⁹ CFU/m²).

TABLE 1 Paecilomyces fumosoroseus Nematicidal active ingredientsControlling (3 × 10⁹ CFU/m²) (dosage mgAI/m²) value (%) − Fosthiazate(300) 0 + Fosthiazate (300) 85 − Imicyafos (300) 50 + Imicyafos (300)100 − Fluopyram (10) 0 + Fluopyram (10) 62 − Cadusafos (900) 50 +Cadusafos (900) 100 − Fluensulfone (400) 0 + Fluensulfone (400) 85 − —0 + — 39

As a result, when treating Paecilomyces fumosoroseus and nematicidalactive ingredients, an excellent controlling effect was exhibited onplant diseases.

Test Example 2

One hundred milliliters of a suspension (1×10⁶ CFU/ml) of Paecilomycesfumosoroseus (strain FE9901, accession number: NITE BP-01876) spore anda prescribed amount of nematicidal active ingredients were treated to apot with a radius of 11 cm filled with Ralstoniasolanacearum-contaminated soil, and well mixed. Thirteen days afterchemical treatment, tomato seedlings were transplanted to this pot. Oneday after transplantation, a Ralstonia solanacearum suspension wasinoculated to the pot by drench, and tomato was cultivated in aglasshouse, at a daytime room temperature of 35° C. and a night roomtemperature of 25° C. Seventeen days after transplantation, the diseaseindex was assessed from the degree of wilting of tomato, and the diseaseseverity was calculated by the following equation, based on the diseaseindex. The disease severity is defined as a disease severity of treatedplants.

Tomato was cultivated in the same manner as described above, except fornot treating Paecilomyces fumosoroseus and nematicidal activeingredients, and the disease severity was calculated. The diseaseseverity is defined as a disease severity of untreated plants.

The controlling value was calculated from the disease severity oftreated plants and the disease severity of non-treated plants.

Disease index

-   -   0: no symptom of disease is found    -   1: wilting of the shoot apices is observed    -   2: wilting of the leaves is observed    -   3: the whole plant is wilted    -   4: the plant withers and dies

Disease severity=100×[{1×(number of plant with disease index of1)+2×(number of plant with disease index of 2)+3×(number of plant withdisease index of 3)+4×(number of plant with disease index of4)}/{4×(total number of plant)}]

Controlling value (%)=100×(A−B)/A

A: disease severity of untreated plants

B: disease severity of treated plants

The result is shown in Table 2. In the table, “−” represents the case ofnot containing Paecilomyces fumosoroseus, and “+” represents the case ofcontaining Paecilomyces fumosoroseus (3×10⁹ CFU/m²).

TABLE 2 Paecilomyces fumosoroseus Controlling (3 × 10⁹ CFU/m²)Nematicidal active ingredients value (%) − MEP(10 mgAI/m²) 0 + MEP(10mgAI/m²) 75 − Bacillus firmus 0 (6 × 10⁸ CFU/m²) + Bacillus firmus 50 (6× 10⁸ CFU/m²) − — 0 + — 25

As a result, when treating Paecilomyces fumosoroseus and nematicidalactive ingredients, an excellent controlling effect was exhibited onplant diseases.

INDUSTRIAL APPLICABILITY

Plant diseases can be controlled, according to the present invention.

1. A composition for controlling plant diseases comprising Paecilomycesfumosoroseus, and one or more nematicidal active ingredients selectedfrom the group consisting of Group (A1) and Group (A2): Group (A1):group consisting of aldicarb, thiodicarb, carbofuran, carbosulfan,dimethoate, fenamiphos, imicyafos, fensulfothion, terbufos, fosthiazate,dichlofenthion, isamidofos, fosthietan, isazofos, ethoprophos,cadusafos, phorate, thionazin, triazophos, diamidafos, phosphamidon,fluensulfone, and MEP (fenitrothion); and Group (A2): group consistingof Harpin protein, Pasteuria nishizawae, Pasteuria penetrans, Pasteuriausgae, Myrothecium verrucaria, Burkholderia cepacia, Bacillusamyloliquefaciens, Bacillus firmus, Bacillus subtillis, Bacilluspumulis, Trichoderma harzianum, Verticillium chlamydosporum, andBacillus megaterium.
 2. The composition for controlling plant diseasesaccording to claim 1, wherein the nematicidal active ingredients are oneor more nematicidal active ingredients selected from Group (A1), and theratio of the number of Paecilomyces fumosoroseus to the amount of thenematicidal active ingredients selected from Group (A1) is number ofbacteria (CFU):amount (g)=10⁷:1 to 10¹⁷:1.
 3. The composition forcontrolling plant diseases according to claim 1, wherein the nematicidalactive ingredients are one or more nematicidal active ingredientsselected from Group (A2), and the ratio of the number of Paecilomycesfumosoroseus to the amount of the nematicidal active ingredientsselected from Group (A2) is number of bacteria (CFU):number of bacteria(CFU)=1:10⁵ to 10⁵:1.
 4. A method for controlling plant diseasescomprising applying an effective amount of the composition forcontrolling plant diseases as defined in claim 1 to a seed, a plant, orsoil for cultivating a plant.
 5. A plant seed treated with an effectiveamount of the composition for controlling plant diseases as defined inclaim
 1. 6. The composition for controlling plant diseases according toclaim 1, wherein the plant disease is a soil-borne disease.
 7. Thecontrolling method according to claim 4, wherein the plant disease is asoil-borne disease.
 8. The plant seed according to claim 5, wherein theplant disease is a soil-borne disease.